Part:BBa_K2259093

SynORI base vector 2.0 insert

This composite part is designed to replace the base vector's Part:BBa_I51020 insert which is no longer available because of the ccdB part containing construct withdrawal.

Vector backbone which does not contain this insert can be [found here.]

These combined sequences result in the new Base vector 2.0, which instead of ccdB poison uses blue-white screening to select for correct colonies when building a custom plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 462
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

Usage with SynORI (Framework for multi-plasmid systems)

About SynORI

SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!

This part in SynORI

Engineering an improved, functional base vector 2.0 was crucial for the SynORI framework because building a synthetic origin of replication required an empty biobrick site and no origin of replication in the backbone. One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transferred to another plasmid location and biobricks are then free to use for other projects required.

See the [http://2017.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania 2017 team wiki] for more insight about our synthetic origin of replication (SynORI).